I want to transfect CHO cells to produce a protein. There is several kinds of CHO cells. I need to use the best one for high yield protein production.
Dear Abbas,
can u tell me what kind of cell culture you want to work with. I mean suspension or adherent? Ar ae u willing to do production at t industrial scale or just want to overexpress it as a need of short academic project? I have worked for 3 years on CHO cells but to give you a concrete answer , I need above info.
Hai Abbas,
We produced our target protein with a roller bottle system of Greiner. In our set-up with our plasmid and gen-specific promoter we had the better results in Hela S3 Cells when compared with CHO-k1 or HEK293. Find attached a result with GFP-protein which we used, based on it simple ready-out, as test-protein.
Dear Pant,
I use RPMI-1640 with 5% FBS and glutamine for adherent CHO cells. It is a academic project. I knew there is some kinds of CHO cells and wanted to know which one is better.
Dear Ruiters,
I used CHO cells to produce 3 Fc-tagged proteins. IFA results and also western blotting were great, but they didn't produce as I expected. Protein extraction is set and I was looking for a answer to low yield production.
Of course it will be great to find better substitution for CHO cells, and if there be a suspension line suitable to produce human source proteins, it will be great.
Thank you for your attention
We used to use CHO cells to produce antibodies, but we get a LOT more protein using HEK cells. FreeStyle 293-F cells from Invitrogen grow in suspension. I get 50 - 100 ug of antibody per mL of culture.
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