I am curious what your favorite cloning method is and why. Is it Gateway cloning, Gibson assembly, Golden Gate cloning or 'cut and paste' assembly via restriction enzymes and ligase? Or a completely different approach?
I'm quite fond of the recombineering-based strategy called PaperClips developed by https://www.researchgate.net/profile/Maryia_Trubitsyna
Thanks, Matthew!
In a textbook (Biochemistry, 2nd edition by C. Grisham and R. H. Garrett) I found the brief description of so-called calcisomes. These are vesicles/organelles that store calcium, similar to the...
02 March 2018 969 2 View
Steroid hormones are usually transported in the blood stream by carrier proteins. At their target cells they are released and can then either bind to surface receptors or diffuse through the...
02 March 2018 5,304 6 View
According to the principle of energy conservation it is not possible to create or destroy energy. Remarkably, PubMed database queries using the terms (mitochondria AND "energy generation") or...
05 June 2016 6,437 17 View
02 March 2015 5,709 8 View
10 November 2014 9,636 3 View
Cold Spring Harbor Laboratory offers a free pre-print server for the biology field (http://biorxiv.org/), similar to arXiv.org which is a pre-print server for manuscripts in the area of physics,...
09 October 2014 396 7 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...
03 March 2021 401 4 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View