What is your favorite cloning method?

More Christian Q. Scheckhuber's questions See All

What is the nature of calcisomes?

In a textbook (Biochemistry, 2nd edition by C. Grisham and R. H. Garrett) I found the brief description of so-called calcisomes. These are vesicles/organelles that store calcium, similar to the...

02 March 2018 1,125 2 View

Some questions on steroid hormones. Can anyone help?

Steroid hormones are usually transported in the blood stream by carrier proteins. At their target cells they are released and can then either bind to surface receptors or diffuse through the...

02 March 2018 5,484 6 View

Isn't it wrong to say that mitochondria generate or produce energy?

According to the principle of energy conservation it is not possible to create or destroy energy. Remarkably, PubMed database queries using the terms (mitochondria AND "energy generation") or...

05 June 2016 6,577 17 View

How do you inhibit protease activity during isoelectric focusing?

Protease inhibition is essential for the quality of isoelectric focusing/2-D SDS-PAGE experiments. I recently used the 'Protease Inhibitor Cocktail for use with yeast and fungal extracts' from...

02 March 2015 5,850 8 View

Is anyone familiar with the use of CMAC-based vacuole markers in filamentous fungi?

I recently tested the components of the "Yeast vacuole staining kit" from Life technologies for staining vacuoles in Penicillium chrysogenum. Carboxy-DCFDA and MDY-64 (both use the GFP...

10 November 2014 9,810 3 View

What is your opinion on the free online archive and distribution service bioRxiv?

Cold Spring Harbor Laboratory offers a free pre-print server for the biology field (http://biorxiv.org/), similar to arXiv.org which is a pre-print server for manuscripts in the area of physics,...

09 October 2014 548 7 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,987 1 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View