I'm trying to grow scenedesmus obliquus, chlorella vulgaris, scenedesmus sp and chlorella sp.
On Monday I inoculated 4 mL of inoculum in 4 mL of BG11 medium (prepared as 1 mL of BG11 and 999 mL of miliQ water) in separately falcon tubes. I put also agitation. The final mix was very green.
However, on Wednesday, the falcon was so clear and all the microalgae culture was dead.
I'm doing something wrong with the way of growing the microalgae?
Dear Noelia García Vázquez
Usually, the inoculum percentage varies from 5% - 20%. In most cases, it is 10%. But, in your case, it is 50% v/v. It is plausible that all nutrients you added in the medium have been exhausted; eventually, it reached its starvation phase and followed by death. You can calculate the F/M ratio (food to microorganism) and compare it with the literature for clarification. Therefore, as per my point, due to deficiency in nutrients, cell death happened.
I hope it helps you.
Thanks
Chandan
My website: https://www.chandanw2e.com/
Dear Noelia
I disagree with Chandan because i don't think that your algae are dying because of nutrient depletion, because in your medium you don't have any.
It's a matter of fact that you are cultivation in milliQ water, because you are diluting your BG11 1000 times, so all the nutrients are almost zero.
For green algae you should inoculate in not diluted BG11, in that way you will not encour in any nutrien starvation.
If you want a "lighter" medium i suggest a BBM medium.
Best regards.
Dear Noelia,
It seems to me that you diluted your medium too much, as noted also by Giovanni. You probably caused an osmotic schock to your algae...
Good luck with the next attempts
Marco
Dear Noelia García Vázquez
Your media is to much diluted, may be the region of nutrient starvation, You can use the Rippka et al., 1979, BG-11 N+ media for your desired algal samples. I am also working on the members of chlorophyceae (Chlorell, Scenedesmus, Monoraphidium, Chlorococcum, and Haematococcus) all these green algae are best growing in the BG-11N+ medium. Here is the attached file of modified Rippka et al., BG-11N+ media. This can help you very much.
Here is the two way of Inoculum (Algae culture) selection for inoculation
1. a- 1% inoculum for culture maintenance.
b- 2% inoculum for growth experiment.
c- 5% inoculation for biomass production
2. N1V1=N2V2 method
If you have any confusion, you contact me without any hesitation on my Email ID. [email protected], [email protected] or Whats app No. for online call +91-7607013814
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