What is the molecular wight of the laccase enzyme from T.vers? (Assuming I don't have a positive control and taking isozymes into consideration).
using a general bio-rad protein ladder, which range is most relevant?
Entries in the BRENDA database for this enzyme give it a molecular weight of between 64 and 110 kDa. Use high-range markers.
I will suggest you use a prestained broad-range molecular weight marker (17- 124 kDa) by sigma. The Molecular weight of the enzyme varies with different strain or variance of the Trametes sp. A search through review article on laccase establish the Mol. weight to be between 60 and 112 kDa.
Hi Noam! we've found two variants: one close to 87 kd and the other one arround 140 kd. I know the 140 one looks too heavy... but it definitely had activity against DMP. Maybe it is a polymer. We used the Pierce Blue Protein Molecular Weight Markers (Thermo Scientific, Rockford, USA) I attach the file in case you want to take a look at the gel.
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