I'm trying to produce laccase using Trametes versicolor and according to the literature i'm not sure if I should use ABTS (2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid)) or syringaldazine.
What is the use for each of them?
Hello, Syringaldazine and ABTS have different chemical structures but both can be used for checking Laccase activity, they both acting by different mechanisms.
Syringaldazine + O2 + Laccase > Oxidized Syringaldazine + 2H2O.
ABTS + Laccase > ABTS + -> ABTS 2+
Oxidized ABTS radical and Oxidized Syringaldazine were green. Measure OD at 530 nm.
I will recommend to use ABTS.
Good luck!
In biochemistry, 2 ABTS is a non-specific peroxidase substrate used to observe the reaction kinetics of enzymes like laccases. Syringaldazine, on the other hand, is a non-auto-oxidizable laccase-specific compound. So the difference is really in their specificities, apart from the chemistry of the compounds.
Hello, Syringaldazine and ABTS have different chemical structures but both can be used for checking Laccase activity, they both acting by different mechanisms.
Syringaldazine + O2 + Laccase > Oxidized Syringaldazine + 2H2O.
ABTS + Laccase > ABTS + -> ABTS 2+
Oxidized ABTS radical and Oxidized Syringaldazine were green. Measure OD at 530 nm.
I will recommend to use ABTS.
Good luck!
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