I am trying to assist a researcher who wants to confirm a protein ID she has from a western blot. She'd like to run just the western band, and rather than the full in-gel she'd like to run after a Western detection to verify. However, it's a very low abundance protein and I am concerned about sensitivity. Does anyone have any advice about the working sensitivity range you typically observe from an on-membrane digestion? Or even a recovery estimate?
Any other tips or suggestions also welcome.