T-test or one way ANOVA in RT-qPCR gene expression?

Hello, I checked the expression of several genes from different strains and I obtained the values of the relative normalized expression of each gene from each strain compared to the relative gene...

28 April 2019 3,598 3 View

How do i set up Biological replicates using CFX Maestro Software Bio-Rad?

Hello everybody, I am performing gene expression analysis using the Bio-Rad CFX maestro Software. I have different strains which I set up as "biological groups". In different plates I put my...

13 January 2019 5,685 2 View

How much sample should be used in a no RT control?

I would like to know when running a no rt control for qPCR should the amount of template used be the same as the amount used to make cDNA or the amount of cDNA used in the qPCR. I use 100ng of RNA...

15 October 2018 7,022 3 View

Replacing a standard curve reagent in validated assay?

17 October 2016 5,744 6 View

Copan eNAT swabs for microbiome work?

Hi all, So I've been working up a lab protocol for extracting DNA from oral and nasal swabs for 16S sequencing. Just when I thought I'd made my decision on swabs to use etc I get a recommendation...

26 September 2016 4,651 2 View

Optimising a competitive ELISA for sensitivity?

20 June 2016 8,127 10 View

Does the presence of trypsin degrade cytokines?

I have samples of frozen tissue supernatant that are being analyzed for cytokine production. However, the cells have been treated with trypsin. Will this affect the concentration of cytokines once...

15 June 2016 2,381 6 View

How do I solve my problem with my ChIPs?

01 September 2015 287 5 View

Why do I have flaky DNA clumps upon Ethanol precipitation?

I did a large scale (6 L) extraction of DNA using Phenol/CIA and PEG precipitations. The step before the ethanol precipitation is CIA extraction. After adding the 2.5X ethanol the DNA looks...

19 March 2015 9,510 2 View

Why is my fusion protein expressed in the nucleus?

I recently made a fluorescent fusion protein that I expected to be cytoplasmic but instead localises to the nucleus. I have run the amino acid sequence through online NLS indicators 'NucPred' from...

02 January 2015 8,223 5 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

Which root in this tap root system, in the picture attached, should be used for root sectioning in order to study the root anatomical details?

There are numerous roots in this tap root system. Kindly suggest or share a research paper where I can clarify the doubts as to which root should be chosen for sectioning and for further root...

14 February 2021 1,816 3 View

What is the relationship that determines the depth of the mechanical cultivator with the age or size of the weed For crops planted in lines?

the great depth of the cultivator may lead to passage under the roots without killing them and may give negative results to break up the soil below the roots, increasing their activity, especially...

03 February 2021 3,198 7 View

Why do high doses of humic substances inhibit the growth of corn roots? Is there a toxicity effect?

Length, volume, surface area and dry mass of corn roots treated with humic substances increased up to 13 liters per hectare and decreased with higher doses.

02 February 2021 2,190 4 View

Does the subsoil have an effect on the spread of weeds whose roots are more than 35 cm deep?

For weeds whose roots deepen more than the level of subsoil, they may be affected by this layer, for example, determined by height and growth, as well as limited to flower production and seeds.

01 February 2021 1,940 4 View

Can you help me find a good, new (2009 and above) resources to get some informations and basics about plant systematic?

Hi, I hope you are doing well I'm a college student interested in botany and everything related to it. Lately I've been so into classification of plants and I don't know how to find a resource. I...

01 February 2021 1,549 5 View

What is the magnitude of yearly rhizopdeposition in tropical forests?

Hi all, I am trying to find reference values on annual soil rhizodeposition rates for different kinds of forests, with a particular interest for tropical rainforests. Do we have an idea of the...

26 January 2021 4,278 4 View

How to use plant root tissue for experimental analysis?

Hello everyone, I am working basically on plants (hydroponic culture). I want to estimate biochemicals and gene expression pattern of root tissues. My question is that how to harvest the tissues...

20 January 2021 4,443 3 View