I am going to collect saliva from 30 persons at a remote area. This might take up to a week before returning to the lab. Can i place the samples in a freezer at a household there during that week? The saliva will be analysed for genomic and mitochondrial DNA extraction, methylation, in addition to Vitamins B test. It will be collected without a swap but by spitting in a 15mL Falcon Tube.
What are the biological materials which can be harmed in the saliva without suitable storage?
I think you must keep it in incupater at the same body temperature. i.e about 37 Co
Regards
I have used stored saliva for metagenomics and ELISA. I used 1:1 dilution in Ambion RNALater and stored in -80 till transport at room temperature. The results are reliable. DNA Genotek has a saliva collection vial pre loaded with a nucleic acid storage reagent, I think that is very convenient.
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