I've used 15% polyacrylamide for synthetic ssDNA oligonucleotid for secondary structure analysis. The gel was stained with silver staining method but it was very very dark. Is 20% polyacrylamide gel a good alternative for better result?
With denaturing gels 20percent gel is good for oligos 8 - 25 bases and 10 percent is good for 25-35 mers so it depends on the length of your oligo and also if you are running native non denaturing gels possibly also on the sequence of the oligo in part but andrew is right that the detection will continue unless you stop it