What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

How to accurately determine the total content of carbon in ammonia-ammonium carbonate mixed solution?

How to accurately determine the total content of carbon in ammonia-ammonium carbonate mixed solution? When I am doing the experiment, I find it is difficult to accurately determine the total...

04 January 2021 2,694 1 View

Which is better in determining the effectiveness of artificial licks for wildlife detection in forested areas?

Hello, I'm planning for my future research study on artificial salt lick. The study site that I've selected for this study has been confirmed with no presence of natural salt lick, thus I plan to...

02 August 2020 5,609 1 View

Should I use the Nafion 211 as proton exchange membrance in microbial fuel cell?

I'm currently using Nafion 211 for the proton exchange membrane in my microbial fuel cell experiment. I found lots of articles recommended to use Nafion 117 instead. My experiment was failed,...

11 November 2019 9,326 3 View

How to calculate effect size in R with Metafor package?

07 October 2019 8,027 3 View

What kind of cells are suitable for cytotoxicity test of nanoparticles?

Hi everyone, I'm currently working on bone-targeting polymeric nanoparticles for treatment of osteoporosis. Just wondering what kind of human osteoblast (or other bone cells) are best suitable for...

03 October 2019 9,077 2 View

Where i can download data of protein/gene interaction networks, preferably in matlab format?

Dear friends, i am a researcher on numerical computation, especially interested on PageRank computation. Now, I am teaching in a agriculture university, thus i want to do some applications to...

27 August 2019 7,598 4 View

How to stain T cells for Ki67/Granzyme B and other surface activation markers together?

Could anyone help me with staining T cells for surface activation markers (CD69) and Ki67/Granzyme B at the same time? The Ki67 staining protocol requires 70% ethanol treatment which might be too...

13 August 2019 7,513 4 View

What is the optimal diameter of the beads for extracting fungal DNA?

I want to extract fungi and bacteria DNA from skin swab. Some articles used two 5mm steel beads per sample, some used 0.4-0.6mm ceramic beads per 0.5g sample. And other diameter for beads were...

05 August 2019 6,471 1 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View