Dear colleagues,
I would like to ask if there is a Standard, guide, manual or well-explained procedure to do the "pot experiment", also known as "plant growth experiment" (there is another name for this experiment?). Additionally, if there is a special one for maize growth, that would be great.
In fact, I read several articles where this experiment was used, however, in none there was mention of a standard, protocol or manual. I am kind of surprised about it, as this procedure seems quite important.
If you have any tip, article, thesis or any trustable document that I could use to guide me, that would be great. I plan to do this experiment with a collaboration, however, I need to know better the procedure to plan ahead.
I thank you all in advance for your time and attention.
Have a great week.
Kind regards
It must have replication and rotation and there must be 6 pots per replicate and at least 3 replicates and equal environmental conditions :)
Dear, Luanna Moura. Pot experiment refers to experiments conducted in pots rather than in the field. As Andrew Paul McKenzie Pegman wrote, there should be 3 to 6 pots per replicate and 3 to 4 replications per treatment.
The attached paper has followed the pot experiment in maize. I hope it helps you. Good luck
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Luanna, I expect that there's a lot of literature on potted maize trials, and I suggest you compare their experimental designs with the facilities used and the variability that was encountered. There are some principles to apply to your own growing conditions, rooting media etc. (which I believe in having done many greenhouse trials with potted eucalypts in Portugal - I have no experience with maize). Try to do pilot studies to understand your own total variance, and to get an idea of how to partition it in your experimental designs. Personally I'd do several smaller trials rather than fewer bigger ones, so that you can observe the variation between trials, and similarly I'd keep the designs relatively simple - eg. randomised complete blocks. Finally a couple of very basic points - make sure you have plenty of error degrees of freedom, and if there is any mortality make sure that the number of plants per plot is sufficient to avoid missing values or inflating the error variance.
Philip
Luanna Moura you might find this article helpful.
Article Techniques for experiments with pot plants
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