I have to extract DNA from a small testis biopsy and we tried to homogenise the tissue with a 5mm bead in lysis buffer. However, the tissue remained intact.
Is it better to do it when frozen in lysis buffer?
Or would snap freezing the tissue and then lyse it with the bead in dry be better for further DNA extraction?
Any suggestions would be kindly appreciated.
Hi Sofia, whenever we had problems with DNA extraction because the tissue remained intact was because there wasn't enough proteinase K concentration in the lysis buffer. As for snap freezing the tissue, we always froze the tissue in isopropanol first.
Covadonga Vara
Hey Cova!
Which concentration were you using and which size of tissue?
Also do you do the lysis overnight or?
I have now tried lysing the tissue with PBS buffer and a bead before hand and it seems to work nicely.
Did you ever try this?
- Badges
- Science topic
- Similar topics
- Phytochemistry
- Extracts