Problem in detection of a specific gene & elimination of primer dimer in qpcr?

In our lab, we are trying to do real time PCR for some genes. One of them has a size of 85 bp. Whatever we do, we had an amplification in negative control. When we run agarose gel, it always a...

14 February 2021 9,750 11 View

How can critical thinking be developed in scientific research?

Dear researchers, As I know, critical thinking is an essential feature that should be in scientist. Can you tell me how this skill can be developed? or how do you gain it from your long research...

16 September 2020 2,608 4 View

Online miRNA target prediction tool and related- down stream pathways?

There are many miRNA target prediction softwares, can you help me from your experience which one is the best? What are the consideratios I must take? because I have no experience in it...

02 September 2020 6,714 5 View

Is there a substitute to InhibitEX buffer in Qiagen Stool DNA extraction Kit?

I have a DNA extraction kit and would like to know if there is a way to make my own InhibitEX buffer (or something similar with the same function) to remove PCR inhibitors commonly present in...

23 August 2020 3,079 2 View

Fault analysis with Simulink- abnormal response after fault clearance?

The attached simple model with SIMULINK after clearing the fault, the Simulink is having abnormal responses. Bus voltage V3 is oscillating. anyone facing that issue?

09 April 2020 2,882 3 View

Can I appeal against a journal editorial decision?

Can I appeal against a journal editorial decision?

28 November 2018 8,076 50 View

How to convert Ti (integral time) and Td (derivative time) to I and D parameter in function block parameter for PID controller (SIMULINK)?

I need help on my problem. I managed to tune my PID controller using Ziegler-Nichols continuous cycling method and able to determine my Kp, Ti (integral time) and Td (derivative time). But the...

22 May 2017 5,641 8 View

What is the difference between generalised additive model and polynomial regression?

13 June 2016 6,758 5 View

How can I put cluster labels in the Gaussian Mixture Model (GMM)?

I used GMM to cluster my data. It has 4 dimensions with 3 clusters. The GMM ran very well, and I got the mean and covariance matrix for each cluster. The problem is I don't know which label...

15 October 2014 7,213 5 View

Where can I find researchers interested in education reform and health problems affecting students caused by problems in education?

I'm extremely interested in issues concerning students' heath in educational settings such as stress levels in class rooms and how standardized testing affects drug use on campuses. I would like...

27 July 2013 7,421 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

In an eye with Tyndall, does each dot correspond to an individual cell?

When you observe the presence of Tyndall in an inflamed eye: Does each of the small floating dots correspond to an individual cell or to an aggregate of cells? Does a conventional slit lamp have...

27 February 2021 6,326 2 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

What could be the reason for not having further low chi square value (better agreement) in my refinement result?

I have tried every combination of parameter changes and after that i have a poor chi square value. I can't get it lower any further. I know there have been a lot of discussion about Rietveld...

26 February 2021 8,480 3 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

I have need of XRD graph analysis software for phase identification. Can you please provide links for software tools which can be downloaded?

i am trying to download x'pert high score software, but can't download it. please do suggest software for phase analysis

24 February 2021 5,687 4 View

Why the CHi^2 is close to zero with a bad fitting but the Rp and Rwp are still high?

I am refining my XRD data using in FullProf program. The intensity of the modulated X-ray peaks is almost flat. I tried to adjust the peak intensities but unsuccessful. What is the reason? I guess...

23 February 2021 2,304 4 View