Neha ..

4 Questions 10 Answers 0 Followers

Questions related from Neha ..

What is the significance of binding constant in fluorescence quenching?

Are the binding constant and binding affinity is the same thing in both fluorescence quenching or fluorescence increment? What is the meaning of getting high or low binding constant (kB), derived...

28 April 2023 7,739 2 View

What is the significance of blue sift in peak in ligand-DNA interaction?

I worked on ligand -Nucleic acid (DNA) interaction for this I have done UV-Visible Spectroscopy of my synthetic chemical compound (Ligand) with the DNA. I did titration of the ligand with...

26 August 2022 7,598 2 View

How to ligate small size insert of 33bp into vector?

I need to clone my small size insert of 33bp only (in vitro anneled duplex oligo) into Psicheck2 plasmid (6.7 KB) by single digestion with NheI, but my ligation is getting failed every time. I was...

08 November 2021 3,552 10 View

I need help for PCR amplification of Plasmid?

My Plasmid is about 64 kb having a T7 Promoter which I need to amplify. Can I use this plasmid directly as a template in PCR reaction using T7 primer? Can this amplification is possible with only...

07 September 2021 5,561 8 View