Is my initial AP FRET set up suitable?

Hi all! I want to use acceptor photobleaching FRET to look at a potential interaction between two proteins of interest. We have a Leica SP8 in my lab that I have used to try and get an idea if...

09 March 2020 2,349 2 View

Is serum diluted for clinical human Anti-idiotypic ELISAs?

I am working on an anti-idiotypic ELISA for human clinical trials and would like to know if human serum is diluted in these assays or used neat. My ab of interest is an IgG4.

01 August 2018 4,708 4 View

Housekeeping proteins/loading controls for a Western Blot of E. coli cell lysates?

18 July 2017 7,679 5 View

What's your favourite mCherry antibody for immunoprecipitation (IP)?

15 January 2017 5,879 3 View

Has length of survival of lab strains of Drosophila changed over the last 50 years?

I am looking for as many different survival curves for D. melanogaster as I can collect. Have many from the literature, but if anyone has survival curve data that they are willing to share that is...

24 August 2016 7,472 1 View

What is the best way to measure glyphosate?

I am looking for either a lab to do the testing or a relatively simple-to-use kit to do it myself. Can it be done on whole body tissues? What quantity of tissue is required? How expensive is this...

03 February 2016 1,367 3 View

Can anyone offer advice on sarkosyl insoluble tau preparations?

25 January 2016 6,634 1 View

Does anyone know if Santa-Cruz Biotechnologies CRISPR/Cas9 plasmids can be transfected with reagents other than the UltraCruz Transfection Reagent?

03 November 2015 5,360 2 View

Does anyone know if CypHer5 mono ester dye is stable after fixation?

I'm hoping to use CypHer5 mono ester dye to label bacteria, then infect cells with the labeled bacteria. I need to do a fixation step before microscopy, and I was wondering if the dye will be...

18 June 2015 2,581 2 View

Can anyone help me solve EMSA competition, shift and supershift problems?

I am trying to do EMSAs and I am using a consensus and mutant oligo specific for my protein which I ordered from Santa Cruz and then a custom biotin labeled oligo (I have annealed sense and...

14 December 2013 2,352 8 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View