Too low protein concentration for Western Blot - any advice for polyacrylamide gel method?

Currently have punched mouse brain samples of different regions that are of too low protein concentration for Western Blot. Has anyone used the polyacrylamide gel electrophoresis method to detect...

09 January 2021 3,955 3 View

Western Blot: any reasonable alternatives or troubleshooting advice for punched samples?

Hi, I currently have punched mouse brain tissue separated by brain region (using the cryostat) and the initial plan was to do Western Blot analysis on the punched tissue. We already have the...

01 January 2021 8,884 1 View

Does anyone have experience or suggestions on the polyacrylamide gel method of protein purification and measurement for punched samples?

Hello, I currently have sliced and punched mouse brain tissue using the cryostat and I wish to analyze it for the concentrations of different dopamine receptors. We initially desired to use...

01 January 2021 9,455 2 View

How to insert a line of median into scatter plot in SPSS? Multiple variables possible?

Hi! I am using SPSS chart builder to do grouped scatter plots. In the first scatter, I have two groups on the x-axis. I want to add a line of median to each group for better comparison. How can I...

17 November 2020 8,693 3 View

Economic evaluation in mental health services / DataBASE to use?

Hi! I am conducting a master thesis in the field of economic evaluation and mental health services as systematic literature review. Now I consider what databases to use. I have been thinking to...

09 September 2020 2,010 3 View

Can I use in a meta analysis case reports and rcts? And what calculation Do you raccomend( proportion, relative risk....)?

21 August 2020 5,190 3 View

Where can I find credible statistics for social media platforms?

I wonder where I can find credible statistics about patterns of using social networking sites that can be used as a reference in an academic thesis, preferably downloadable. Thanks

08 May 2020 4,932 5 View

Where can I find research papers or data concerning Demographic and Socioeconomic Characteristics of Immigrants and Refugees ?

Hello Everyone, I do hope that all of you are doing well and staying safe amid the COVID-19. I'm trying to find research papers or data that gives a global view about the difference in the...

03 April 2020 4,157 1 View

Why can't I see collagen in SDS-PAGE?

I try to resolve collagen on SDS-PAGE. My goal is to resolve the collagen extracted from skin scaffolds. I use ThermoFisher Bolt mini gels. I can see the protein ladder migrating and also I can...

15 March 2020 9,906 8 View

Your opinion on pulsed field electrophoresis instruments?

Hello, I am interested in running an experiment requiring pulsed field electrophoresis. The primary samples will be budding yeasts chromosomes and minor set of samples with chromosome sizes up...

30 October 2019 7,068 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View