some hydrocarbons such as decane, dodecane and tetradecane in my samples are dominant(20%) ,I want to know the roles of these compound on algae?
Are these algae harmful or not as a food resources for human?
These even-numbered hydrocarbons would be extremely useful for fuel, if they are really present in your algae. How did you extract them from the algae? It is possible that your extraction solvent may be the source. If you extract your algae with chloroform or diethyl ether there may be no C10, C12 or C14 present.
many thanks dear David
transestrification of my samples carried out during one step without lipid extraction and my solvent were acidic methanol, normal saline and hexane.
i spent a lot of time to find papers about the effects of these components on humans, but there was noting.I read these components are in plants and these plants uses as food sources daily. so that their amount of them should be important. unfortunately information in this is very small or i couldn't access to beneficial sources.
if you have information or paper in this field, i am happy to guide me?
best regards
Your hexane is the suspect. Unless it is GC grade (distilled and meant for gas chromatography) it may well contain octane and its cousins decane and dodecane. These heavier cousins should have been left behind by distilling hexane away from them through a tall glass column, thus these higher molecular weight compounds remain behind in the pot.
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