Hii...

Poly -L-Ornithine is coated in plates to promote cell attachment and this could be used for a variety of cell types...in case of neuronal cells ,collagen works best and oligodendrocytes ,astrocytes doesn't require coating..Bovine fibronectin also enhances cell attachment in cell culture plates

Thanks! Do you know if the adhesion can be optimal if you coat plates with fibronectin only (i.e. without Poly-L-ornithin?)

also, for human lymphocyte adhesion test, would you use rather human or bovine fibronectin? Thank you very much

hiiii

i wonder is it acceptable to pre-coat culture dishes with poly L-ornithine only without laminin in differentiation of embryoid bodies??

thanks in advance