What is the relationship between RNAseH and DNA polymerase? Both of them are responsible for removing of RNA primers. I want to learn about this two mechanism are related with each other or are they independent mechanisms?
It is believed that family A DNA polymerases are fused proteins of DNA polymerase and flap endonuclease. Therefore, DNA polymerase I (family A) posesses an unique activity among others DNA polymerase - 5'-3'-exonuclease activity. RNAse H belongs to another exonuclease family, however, there are fusion proteins of RNAse H and DNA polymerase, e.g. reverse transcriptases. In eukariotic replication Okazaki fragments are hydrolysed by FEN-1 (flap exonuclease), while DNA polymerase delta (family B) fills the emerging gap. Prokaryotes process RNA primers via DNA polymerase I cleavage (or RNAse H1 hydrolysis) and synthesis by the same enzyme.