How to make ppb level concentration ?

Hi, I've N2 gas cylinder and 100 ppm NH3 gas cylinder. With these two cylinders, I'd like to make a ppb level (1- 100) concentration. Any idea how to do that?

01 February 2021 3,264 2 View

Stimulation system for field recordings?

We have a stimulation isolator (A365). When we put the stimulus and recording electrodes into solution (even with miliq water), some current came out (see attached). The current was gone when the...

26 August 2020 9,139 2 View

What is the proper aldosterone and CORT supplement in ADX mice?

Hi, I am struggling with the stress experiements in ADX mice. i tried 15ug/ml cort in the drinking water for ADX mice, however, the basal plasma cort are much higher than sham controls( 260ng/ml...

21 June 2020 386 1 View

What's the reason for the crossover of illuminated and dark JV curve in CIGS solar cells?

The distinct crossover anomalies are observed in my recent samples. It's explained in the book that there might be a barrier at the interface or in the absorber's near interface region.The barrier...

29 May 2020 675 4 View

How should I hydroxylate tungsten powder for organosilane grafting?

I am having trouble depositing MPTMS/APTES onto tungsten and I am concerned there are not enough hydroxyl groups on the powder surface. So far I have been exposing tungsten to water to hydroxylate...

04 February 2020 4,677 2 View

How to calculate effect size in R with Metafor package?

07 October 2019 8,027 3 View

Could I extract RANK/RANKL/OPG mRNA from peripheral blood samples?

I want to know that whether it is possible to extract RANK/RANKL/OPG mRNA from peripheral blood samples instead of tissue homogenates. Thanks a lot.

07 September 2019 7,431 3 View

How to calculate the area of coverage hole of a disc tessellation?

The locations (centre coordinate x,y) and radii of all the circles are given. How to get the area of the coverage hole (area in green in the figure) ? Radii may not equal to each other. It is a...

11 June 2019 5,127 15 View

For WB, if there were DNA in my protein sample, how can I do?

Hi, everyone, I am working on transcriptional factor, I want to define what protein bind to my specific DNA, after I got the sample that bound to DNA, and use SDS buffer to boil it, I can find the...

24 June 2018 4,582 5 View

How the overall score is related to their subscale scores?

This is the third section of Items Decomposition: How to structure the Scores and Items. This section describes, using the graphical representation, how the score associated with all items in a...

07 June 2018 8,942 4 View

How can I set up a good fit SEM model in AMOS?

I am trying to make an SEM model in AMOS for my dissertation but having some trouble with getting a good fit. I am looking for relationships between self-efficacy reported on a Likert scale and...

26 February 2021 8,642 4 View

Is my GFAP staining in right position?

I was differentiate hESC to astrocyte. In the second week of differentiation, I stained my cell with s100beta and GFAP. I expected that the staining site of GFAP would come from the cytoplasm,...

15 February 2021 8,982 1 View

Mixed ANOVA or Multilevel modeling?

Hi everyone, I'm having some questions about choosing the right test for my experiment and I hope I can find help here! Here are the settings of the experiment: 1. participants are randomly...

14 February 2021 1,028 4 View

How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

How to get the insoluble fraction of PK digestion for mitochondria?

I am trying to check for some proteins if they are located in the inner or outer mitochondria membrane after treating the cells with a neurotoxin. I am using PK to digest the mitochondria, but I...

27 January 2021 5,145 2 View

Any suggestion on self-efficacy scale ?

Dear researchers, I am planning on conducting a research on the relationship between Language learning strategies, by Oxford (1990) and self-efficacy. I have found Morgan-Jinks Student Efficacy...

22 January 2021 8,432 3 View

Where did Mitoprot software go (download version)?

Hi all! I've been using local copy of Mitoprot software for prediction of gene localization. https://ihg.gsf.de/ihg/mitoprot.html I've updated my laptop and now need to download Mitoprot again,...

18 January 2021 4,368 3 View

Does maternal potentiation work?

Has anybody been able to replicate finding of papers that show increased vocalization in mice pups after a brief period of interaction with their dam, also called maternal potentiation? We have...

11 January 2021 558 2 View

How can I selectively repress glucocorticoid receptor translocation to the mitochondria in primary cell lines without altering nuclear GR signaling?

How can I selectively repress glucocorticoid receptor translocation to the mitochondria in primary cell lines without altering nuclear GR signaling? Any thoughts on applicable small molecules to...

06 January 2021 4,324 2 View

How an unusual visual effect at the Monte Carlo casino can be obtained?

At the Monte Carlo casino, I saw an unusual advertising image. It rotated around a vertical axis and seemed suspended in the air; there was no screen behind it. The image was bright and colorful....

03 January 2021 9,119 5 View