Dear friends,
Recently, we use LPS to induce 24h-mouse spesis model to explore the effect of some drugs to splenic Treg expansion. However, sometimes we failed to induce sepsis and splenic Treg expansion after LPS i. p. injection. I asked some friends and they told me that the mice in USA and mice in China have different lethal dose of LPS injection. In addition, they encourage me to use LPS produced in China, which may be a misture instead of ultrapure LPS from sigma. I wonder why sometimes LPS failed to expand Tregs in mice? In addition, may I know the different between LPS from Sigma and beyotimes? May I know some details about LPS subtype and application in experiments.
Yours
Shuoyang Liu
LPS from sigma is:
L9764 Sigma-Aldrich Lipopolysaccharides (rough strains) from Salmonella enterica serotype minnesota Re 595 (Re mutant).
LPS from beyotime(China) is : lipopolysaccharide purified from E. coli O111:B4, which is specific for TLR4 activation or TLR2.
The mice are bought from other labs. The body weight is from 17g-19g. The mice from other labs and from our breeding sometimes are different in response to LPS induction.
Why are you using LPS isolated from different bacteria?
Even LPS isolated from different stains of E.coli will have differences in activation/stimulation of immunity.
You can buy LPS from E.coli O111;B4 from Sigma too.