When we studying the gene expression of any antioxidant genes in different plant genotypes under different levels of stress, what explains the increase or decrease in the level of expression according to the genotype or the stress level?
Is there a genetic basis in that two genetically related people like the same thing (color, music...etc)? Thanks in advance.
03 April 2018 6,699 4 View
Are the nuclei aged irreversibly?
03 April 2018 1,196 4 View
If scientists got some money, what they will do with it?
02 March 2018 1,324 14 View
When you read a question you don't have an answer for, what you will do first?
02 March 2018 8,824 2 View
I investigated the relationship between DNA methylation level and gene expression in different plant species under different affects, and I found it negative in general. I get confused when I read...
02 March 2018 7,991 6 View
I have a procedure involves the using of polyacrylamide, but I don't have it! Can I use MetaPhor instead? What limits the using of MetaPhor instead of polyacrylamide?
02 March 2018 6,390 2 View
We learned that phenotypic value is the sum of genetic value and environmental effects: P=G+E, Where is the epigenetic effect?
02 March 2018 2,309 2 View
Theoretically, larger genes are more susceptible to mutations than smaller genes. Why?
01 February 2018 6,115 10 View
The plant in the attached image is a wild type! It have been grown in pots and all growth conditions were in educate levels. Why does the plant still show symptoms of stress! What is the role of...
01 February 2018 3,258 7 View
In many occasions I have problems with DNA extraction from seeds tissue, especially wild types seeds.I tried different protocols, but i still have invalid DNA! Did any body experienced an...
01 February 2018 2,385 5 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
What is the role of oxidative stress in toxin-induced carcinogenesis? I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from...
02 August 2024 3,174 1 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....
26 July 2024 1,887 0 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View