How do I prepare cold buffer with potassium phosphate and EDTA, for the procedure mentioned on page 10 of the attached booklet?
Recipes for potassium phosphate buffer of different pH levels can be found here: http://www4.mpbio.com/ecom/docs/proddata.nsf/(webtds2)/194727
"Cold" buffer simply means that the buffer has been chilled. Before using it, stick the buffer in the fridge for a few hours. That's "cold buffer."
Thank you very much for the answer!
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