Mihir Parikh

6 Questions 5 Answers 0 Followers

Questions related from Mihir Parikh

How to identify and locate rat ECG waves?

Attached is the ECG tracing obtained from a rat on which coronary artery ligation surgery was performed. How to identify which wave is what?

18 January 2018 1,569 5 View

How to get good sections of rat heart in cryostat without tissue falling off?

I wash rat heart (left ventricle) 2 times with PBS over an ice bath, dry it with paper towels and then embed it in OCT over cooling bath. Lately, while sectioning in cryostat tissue is falling...

24 May 2017 1,239 6 View

HEPES buffer for SOD estimation.

Does anyone know the exact composition and recipe of the HEPES Buffer for the procedure mentioned on page 9 of the attached booklet.

16 May 2017 3,832 4 View

What is the recipe for cold buffer?

How do I prepare cold buffer with potassium phosphate and EDTA, for the procedure mentioned on page 10 of the attached booklet?

16 May 2017 1,924 2 View

Buffers for ROS assays?

Can we use the same buffer (for example tissue lysis buffer) for estimation of GPx, SOD, and Catalase?

29 January 2017 4,059 3 View

Marker for angiogenesis in cardiac tissue?

In the coronary artery ligation-induced myocardial infarction model in rats, which marker should be used to assess angiogenesis in left ventricle tissue? Should I go for CD31 using IHC technique...

29 January 2017 2,525 4 View