What information can be obtained from a 3D scan in fluorimtric assays?

I have performed 3D scans for fluorescence studies, which consist of contours at the emission wavelength. how to draw useful information from these 3D maps and to understand the dynamics of the...

04 May 2019 8,959 4 View

Is it possible to determine the porosity behaviour of colloidal particles in solution phase or in the form of film?

Actually, the trivial techniques meant for the pore size determination (BET, BJH) cannot be applied for the inquired systems, but I wish to know the porosity trend (whether meso/macroporous),...

08 September 2018 9,104 3 View

Which technique can be used for separating the organometalic powdered catalyst from the organic reactions?

I have prepared a supported catalyst, which is implied for catalyzing certain organic conversion. The catalyst along with reacting precursors are kept under stirring conditions in order to...

06 July 2018 4,205 5 View

How to decide whether the synthesised nanoparticle system is alloy or multimetallic (bimetallic and trimetallic of noble metals)?

Which technique can be used to clarify whether the individual metals are homogenously mixed or are arranged in the bimetallic or trimetallic nanoparticle array?

06 July 2018 8,808 3 View

Do nanomaterials also show the Raman spectra, or just enhance the signal of the materials adsorbed on them?

I want to inquire whether nanomaterials merely act as the substrates for some molecules which are to be analyzed, or they themselves show lines in Raman spectroscopy. For reference, an image of...

06 July 2018 2,626 2 View

Can we use another working electrode for SWASV, instead of thin film mercury electrode?

Usually dropping mercury electrode or Hg modified GCE is used for stripping voltammetry, since it is a conventional or standard process, therefore is it possible to use GCE modified by some other...

02 March 2018 437 0 View

How to prevent the leaching out of thin film casted over glassy carbon electrode?

In order to carry out an electrochemical conversion using thin film modified glassy carbon electrode in acidic medium, leaching need to be overcome. what are the possible modifications that can be...

02 March 2018 8,250 3 View

How does the contact angle of a thin film effects it's catalytic efficiency as a heterogeneous electrocatalysts?

I mean if the material of the film is such that It is hydrophobic with high contact angle value, then whether it lowers the catalytic performances of the film as electrocatalysts in aqueous...

08 September 2017 2,866 3 View

How to calculate the number of electron transfer in the oxidation of amino acids using electrochemistry?

05 June 2017 8,298 2 View

How to characterise the wet gel beads?

how to analyse contents in gel beads using HRSEM, EDS and FTIR, as wet gel bead is not preferred for these sensitive instruments?

31 December 2016 2,335 1 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View