When I examined one-step growth curve experiments for bacteriophages, I mainly saw two experimental setups; one is based on bringing bacteria and phage together and precipitating after incubation and resuspending the pellet while the other is based on diluting after incubation and re-incubating that sample and continuing. After incubation of bacteria with phage, after centrifugation of the mixture, what is the purpose of resuspending the pellet again, forming a new mixture and taking a sample for DLA? I can't understand why the two protocol types have different paths.
Another thing is that I already bring bacteria and phage together and incubate in these experiments but I wasn't sure if I had to put bacteria back into it while making DLA the sample I took to create this one step curve.
Article example for two one step-growth curve protocols;
Article Phenotypic and molecular characterization of two lytic bacte...
Article Phage Biocontrol of Pseudomonas aeruginosa in Water
The two procedures are actually fairly similar except in the first article they harvest the cells to remove unabsorbed phage. The second protocol is much more similar to the standard method traditionally used. But either would be fine.
Dear Michael J. Benedik , thank you for your valuable response and advice.
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