Acridine orange flow cytometry apoptosis analysis?

Hello, I got this result from my experiment. i wanted to learn there is apoptosis or not in my treated cell lines. My protocol is Firsly, i added 4 ul of acridine orange to my treated cells in...

26 July 2020 3,135 4 View

Western blotting bands seems so interesting? what is the reason of that?

19 July 2020 8,726 8 View

How can I solve the high impedance problem of saturated calomel reference electrode?

The reference electrod I use is saturated calomel electrode (3M KCl as the filling solution). I got overload problem while measuring the EIS, so then I checked the impedance of the electrod and...

27 May 2020 7,557 12 View

What can cause cracks on the surface of an alloy after anodic oxidation?

We observe some cracks among the nanotubes which are formed on the surface of the Ti alloy after anodic oxdation. What could be the cause of this problem?

13 January 2020 3,086 9 View

How STZ induced fasting glucose 0ver 350mg/dl sudly decreased to 99mg/dl in 4.weks, and after feedig ad libitum,postprandial b. glucos over 500mg/dl?

STZ induced (50mg/kg. ip.female rat),and fasting glucose is over 350mg/dl after 72.hours, 2. week and 3.week, but in 4. week we measure fasting glucose suddenly, below 99mg/dl, When we feed ad...

08 March 2019 8,546 3 View

Is it possible to transfect NBTII cells?

I am currently trying to transfect NBTII cells (wt) with lipofectamine 2000 in Opti-MEM. I tried several things already; such as splitting them 24h or 48h after transfection (I always changed the...

05 June 2018 1,845 2 View

Higher R square and less significant variables?

Im working on panel data and when I performed fixed effects model I get high r square and less significant variables. All the underlying assumptions of regression are satisfied. Kindly let me...

31 December 2017 6,577 7 View

How can we measure the changing of fluorescence intensity on nanofiber mats? Which procedure or specific instruments we need?

Is it possible to measure it with the help of basic fluorescence spectroscopy?

02 November 2017 625 2 View

Does anyone know how calculate piezoelectric constant at VASP program?

Hi All, I am trying to calculate piezoelectric tensor of tetragonal BaTiO3 with the help of VASP package program, I've written LEPSILON=T in INCAR file, after end of the job, I can get...

20 March 2015 2,164 2 View

How to improve quality of RNA from (wound) skin tissue?

05 December 2014 8,039 4 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Mediation path analysis - Can I run two seperate models?

I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...

27 February 2021 5,681 3 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

How can we estimate sample size for a moderated mediation analysis with more than two mediators?

How can we estimate sample size for a moderated mediation analysis with more than two mediators? I am looking for scripts, macros, syntaxes, that allow us to estimate sample sizes for research...

15 February 2021 6,813 3 View

What does it mean if in mediation analysis the indirect effect is significant but is a larger b value than that of the direct effect?

I understand that for partial mediation to have occured the CI for the indirect effect must not cross zero and the b value for the indirect effect should be smaller than the b value for the direct...

14 February 2021 3,273 7 View

Can we have a model where A affects B which affects C but B is not a mediator?

Please suggest any papers in Information Management area that have the model where A affects B which affects C but B is not a mediator? What is this relationship called?

13 February 2021 5,239 3 View

What is the step by step process involved in interpreting the results of mediation analysis done using PROCESS macro?

09 February 2021 7,377 2 View

Is the test of the H1 hypothesis in mediation analysis total effect or direct effect?

There are three hypotheses and I carry out direct effect and indirect effects with SEM. H1: There is a significant positive relationship between A and B. H2: There is a significant positive...

08 February 2021 9,141 3 View

Using two-tailed test for directional hypothesis?

In my study, I have used Process Macro to test the proposed relationships. The hypothesis is directional and two-tailed test has been deployed. Can anyone please clarify how to justify using...

06 February 2021 3,023 3 View

Experiment or Quasi Experiment Design?

What kind of study design are we talking about? Subjects are randomly assigned to one of three groups. In addition, a moderator and a mediator are collected as continuous variables. Is this an...

15 January 2021 1,809 4 View