Dear Mohd Musaib Haidari , apparently this noise could be caused by astigmatism in the electron beam. Often there are a couple of knobs to adjust that kind of beam aberration. It shouldn´t be a problem but it is better if you ask for help from some experienced user or some technician acostumed to use the SEM.

Astigmatism deforms the appearance of objects, so a point object becomes something like a line... that is what we can see on your image according to your description.

I hope it helps.

Manuel Gómez Thank you for your answer.

Hi Mohd Musaib Haidari , you´re wellcome.

Good luck!

Manuel

Manuel Gómez, thank you, sir. actually, there was an insolating layer between Cu and sample holder. So we solved it with silver paste.

Hi Mohd Musaib Haidari , glad to know. Very often, tiny things like that keep us busy trying to understand what is happening.

Good luck with your research!

Manuel