i am working on HPLC & as i know, LOD is the minimum amount of analyte that can be detected by an exact value but not quantified and LOQ is the minimum amount of analyte that can be quantitatively determined with suitable accuracy and precision. but my ques. is that if we get to know the exact minimum quantity of analyte for detection, then what is the role of LOQ? LOD is also a quantity which we determined...
LoQ is the lowest concentration at which the analyte can not only be reliably detected but at which some predefined goals for bias and imprecision are met. The LoQ may be equivalent to the LoD or it could be at a much higher concentration. you can go through the link:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2556583/
Lod =minimum detectable con centration
LOD=3*S(blank)/(slope of calibration curve)
LOq=smallest amount of analyte can be measured with reasonable accuracy
LOQ=10*S(blank)/(slope of calibration curve)
where S is standard deviation of the blank
- Limit of detection (LoD) (also called detection limit) – the smallest amount or concentration of analyte in the test sample that can be reliably distinguished from zero.
- Limit of quantitation (LoQ) – the lowest concentration at which the analyte can not only be reliably detected but at which some predefined goals for bias and imprecision are met. The LoQ may be equivalent to the LoD or it could be at a much higher concentration.
For more information see this link.
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