I would like to follow a modified starch iodine protocol (Hossan et al). In this protocol I found that I’ve to prepare alpha amylase solution (10 mg/ml) in phosphate buffer (0.02M,pH 7.0) with NaCl (0.006M). Now If I prepare 100 ml phosphate buffer (100 mL phosphate buffer: 77 mL of Na2HPO4•2H2O and 23 mL of NaH2PO4•H2O ), then which amount of NaCl (0.006M) I have to mix?

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