What is the rate of deactivation of messenger RNA molecules after death, in the case of a gene-injected animal for example?

19 April 2025 0 8K Report

The question therefore concerns the loss of cytoplasmic activity after death, in particular as regards to the ribosomal RNA required for the synthesis of proteins encoded by messenger RNA.

Badges
Science topic

More Luc Opdecamp's questions See All

How to calculate the conversion of random copolymerization mixture?

I'm working on the RAFT copolymerization of MPC and NIPAm monomer, but I'm not sure how to calculate the total conversion from proton NMR? Is it an average of conversions of two monomers or just...

10 June 2024 7,430 2 View

Is it frequently done for ornamental /.urban tree commercial propagation to renew the tissue culture from stock plant (outside somatic embryogenesis)?

Looking for description of recurrent culture re-initiation in the commercial forest or urban tree tissue culture propagation (outside somatic embryogenesis) . Especially if flowering is not the...

08 March 2022 9,632 3 View

How do you preserve privacy during model development in Federated ML?

Hi all, I am currently researching the topic of Federated ML in industrial applications. While I understand the idea of keeping all the data in a silo private while model training can be federated...

12 February 2022 6,867 0 View

Does an empirical comparison between MMSE and sparse recovery methods for MIMO channel estimation exist?

MMSE and sparse recovery methods both aim at improving the classical LS channel estimation by injecting prior information. On the one hand, the prior information is learnt over time for MMSE (it...

26 October 2021 9,835 3 View

In a MIMO system, how is an LMMSE channel estimator initialized in terms of covariance matrix?

In MIMO channel estimation, the linear minimum mean squares estimator (LMMSE) yields better performance than the least-squares (LS) estimator. However, it requires knowing the channel covariance...

20 October 2021 6,477 4 View

How can I give my articles to Research Gate without disturbing Journal rights???

I do not want to do anything wrong.

07 July 2021 1,290 13 View

Is any researcher interested in obtaining novel parasites found in gastropods for aquariums distributed by Floridian aquaculture companies?

I have what I believe to be several novel species of parasites, including some type of predatory marine isopod, that infected the Florida aquarium aquaculture supply chain. I have searched...

16 May 2021 4,053 3 View

What geographical scales are suitable for sharing information of economic, ecological, social or cultural interest?

It would envisage communication between soil science and other disciplines on the one hand, and farmers, foresters, industrialists, artisans, traders and local residents on the other.

10 December 2020 6,323 4 View

How to obtain in PTRAC file the nature of secondaries produced in neutron-target interactions ?

I am currently performing a quantitative comparison study between Geant4 and MCNP6.2 that concerns the number and nature of interactions between 14 MeV neutrons and a natural silicon target. If...

14 September 2020 3,028 0 View

How can I filter MODIS active fire data to exclude single fire burning several days?

I am using MODIS MCD14DL active fire data (https://firms.modaps.eosdis.nasa.gov/download/)to monitor fire distribution in my area of interest. I would like to exclude for each selected hotspot...

29 August 2020 8,112 5 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View