Tasaduq Hussain Wani

3 Questions 18 Answers 0 Followers

Questions related from Tasaduq Hussain Wani

How much of Gamma H2AX foci is it possible to be found at basal level of any cancer cell line?

Please exclude the case of melanoma which have higher levels of basal gamma-H2AX. And apart from drugs how much can handling of a cell line affect the foci formation in the same?

20 October 2015 820 2 View

How can we exclude TM regions in a protein sequence, when there are many of them in it?

I have to produce antibody for a protein. Upon analysis I found out that there is presence of a signal sequence and nine TM regions. I am confused about, how to exclude all those TM regions when...

18 November 2013 7,139 3 View

Can anybody suggest a way to overcome degradation or loss of protein after dialysis?

Recently I purified one recombinant protein by urea based method (Ni-NTA was used for binding). Initially before dialysis I had a good concentration of protein, but after dialysis I lost my...

16 August 2013 8,205 10 View