What is the "pore size" of brain tissue?

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Does linking alkenes onto tissue increase OsO4 staining density?

I'm trying to increase the contrast of OsO4 stained brain tissue for x-ray microtomography (a recent scan had very poor contrast). I was wondering if there is any literature precedent for getting...

31 December 2019 9,834 1 View

Does EDC/NHS coupling act on primary amides or only primary amines?

I want to use a coupling reagent (possibly EDC/NHS) to link gold nanoparticles to primary amines and NOT primary amides. There are both primary amines and primary amides in my sample, but I want...

31 December 2019 6,329 4 View

How permeable are expanded tissues (when performing expansion microscopy)?

I am planning on performing expansion microscopy on relatively thick (300 um) tissue slices and doing a post-expansion stain with some nanoparticles. It seems that the expansion process should...

31 December 2019 3,550 2 View

How do I store 1% osmium tetroxide [SAFETY QUESTION]?

I'm currently storing a solution of 1% osmium tetroxide (1 gram OsO4 in 100 mL dH2O) in a glass bottle with a plastic cap under a fume hood at room temperature. However, I am concerned that the...

10 November 2019 5,400 15 View

Are there any plastic embedding chemistries that are compatible with very wet tissue?

I'm working with expansion microscopy and I need my samples to have high stability (esp. thermostability) due to intense light beams that are involved in my application. As such, I am trying to...

09 October 2019 7,178 4 View

What are some proteins found on neuronal membranes that are easily amenable to IHC?

I'm looking for a membrane protein (found in neurons) or other antigen that will provide very strong staining from IHC. I would prefer an antigen that is uniformly expressed across dendritic,...

05 June 2019 8,532 7 View

How to make PFA-fixed tissue volumes permeable to nanoparticle-antibody conjugates?

I'm trying to stain ~ 1 mm^3 volumes of PFA-fixed brain tissue with nanoparticle-antibody conjugates. Although my nanoparticles are 5 nm in diameter, I'm experiencing difficulty in getting the...

01 February 2019 5,806 2 View

Where can I find antibodies against cell-surface biomarkers in honeybee neurons?

I'd like to tag honeybee neurons with antibodies for imaging purposes, so I'm looking for a commercial provider of antibodies which are reactive with cell-surface biomarkers found in the honeybee...

09 October 2018 9,103 0 View

What freeware would you recommend for tracing neurons in a tiff stack?

I've acquired a stack of tiff images from a volume of brain tissue. I'm trying to find an automated program (not semiautomated) which will allow me to trace the neurites within this volume.

08 September 2018 8,370 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

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03 March 2021 8,890 1 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

What is Nano technology with coronavirus helpe the human..and is it nano mterals removal of covid19?

I anwar by adsorption or reaction ..but it is either more method...

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How to compare different concentrations of nanoparticles for characterization from ICP-MS?

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02 March 2021 7,626 1 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

Sharafaldin Al-Musawi

Hi dear Logan Collins

I think the following papers and links can help you;

Best and kind regards.

please download the attachment

Article Physiologic upper limit of pore size in the blood-tumor barr...

http://www.pnas.org/content/111/38/13811

http://cancerres.aacrjournals.org/content/77/2/238

I hope that my answer was helpful for your question.