Logan Collins

10 Questions 2 Answers 0 Followers

Questions related from Logan Collins

How permeable are expanded tissues (when performing expansion microscopy)?

I am planning on performing expansion microscopy on relatively thick (300 um) tissue slices and doing a post-expansion stain with some nanoparticles. It seems that the expansion process should...

01 January 2020 3,667 2 View

Does EDC/NHS coupling act on primary amides or only primary amines?

I want to use a coupling reagent (possibly EDC/NHS) to link gold nanoparticles to primary amines and NOT primary amides. There are both primary amines and primary amides in my sample, but I want...

01 January 2020 6,469 4 View

Does linking alkenes onto tissue increase OsO4 staining density?

I'm trying to increase the contrast of OsO4 stained brain tissue for x-ray microtomography (a recent scan had very poor contrast). I was wondering if there is any literature precedent for getting...

01 January 2020 9,933 1 View

How do I store 1% osmium tetroxide [SAFETY QUESTION]?

I'm currently storing a solution of 1% osmium tetroxide (1 gram OsO4 in 100 mL dH2O) in a glass bottle with a plastic cap under a fume hood at room temperature. However, I am concerned that the...

11 November 2019 5,525 15 View

Are there any plastic embedding chemistries that are compatible with very wet tissue?

I'm working with expansion microscopy and I need my samples to have high stability (esp. thermostability) due to intense light beams that are involved in my application. As such, I am trying to...

10 October 2019 7,282 4 View

What are some proteins found on neuronal membranes that are easily amenable to IHC?

I'm looking for a membrane protein (found in neurons) or other antigen that will provide very strong staining from IHC. I would prefer an antigen that is uniformly expressed across dendritic,...

06 June 2019 8,631 7 View

How to make PFA-fixed tissue volumes permeable to nanoparticle-antibody conjugates?

I'm trying to stain ~ 1 mm^3 volumes of PFA-fixed brain tissue with nanoparticle-antibody conjugates. Although my nanoparticles are 5 nm in diameter, I'm experiencing difficulty in getting the...

02 February 2019 5,902 2 View

Where can I find antibodies against cell-surface biomarkers in honeybee neurons?

I'd like to tag honeybee neurons with antibodies for imaging purposes, so I'm looking for a commercial provider of antibodies which are reactive with cell-surface biomarkers found in the honeybee...

10 October 2018 9,188 0 View

What freeware would you recommend for tracing neurons in a tiff stack?

I've acquired a stack of tiff images from a volume of brain tissue. I'm trying to find an automated program (not semiautomated) which will allow me to trace the neurites within this volume.

09 September 2018 8,463 1 View

What is the "pore size" of brain tissue?

I was wondering about the ECM "pore size" within rat brains (in the context of nanoparticle diffusion). In addition, I would like to know if the fixation/treatment method impacts the tissue's...

06 June 2018 552 9 View