I am running experiments with chlamydomonas reinhardtii. We were using 1% BSA in PBS solution to make the glass cover slide less cell adhesive. I am looking for some better treatment and I came across 0.2%(w/v) Pluronic F127 in PBS (10.1038/nprot.2014.120) treatment. Most of the protocol set for this treatment require washing with PBS afterwards. Would it be correct to assume the following protocol for the glass slide?

I am confused about the washing part because It might remove the pluronic layer from the glass slide. Any input will be helpful.