I want to study aggregation kinetics of Abeta (1-40) by ThT. I would like to know the working protocol for this study. I am using plate-reader based assay.
Due to high flexibility IDPs expected to have higher sensitivity in NMR compared to structured proteins. Is this hypothesis correct? If yes can you suggest a suitable reference for this?
04 May 2019 8,775 2 View
I am studying interaction of zinc with a peptide. Which buffer should I use ? And what is the highest concentration of zinc can used ?
08 September 2018 1,440 3 View
How can we compare stability of two proteins with the help of NMR spectroscopy? what are the traditional NMR techniques that we use for this purpose?
11 December 2017 3,766 4 View
If I design a peptide for preventing aggregation of Abeta 42 peptide, what are aspects should I consider if I want to suggest it as drug for Alzheimer disease. What all are the properties should...
10 November 2017 3,234 2 View
I have a calcium binding protein. To make it calcium free i did dialysis with EDTA containing buffer. But how to make sure whether a protein is calcium free ?
10 November 2016 5,580 2 View
In search of some review article on calcium binding proteins and exploring studies that can be performed on them.
05 June 2016 7,727 1 View
I read about circulating tumor cells and understood that cellsearch (Janssen) is the only FDA approved method that existing for capturing cancer cells and they are based on antigen-anibody...
10 November 2015 4,624 7 View
Why do we generally prefer cells like DH5alpha or top10 for increasing plasmid amount and isolation? Why are we using BL21 for protein expression? Why not DH5alpha?
10 November 2014 3,629 10 View
I want to measure torsional angles from a protein crystal structure. Which is the most suitable software for that. I am not a biologist and not an expert in programming.
08 September 2014 3,786 3 View
I have a protein of 277 aminoacids. I want express a truncated version of that without first 22 aminoacids from N-terminal and also the sequence should start with a cysteine residue. Is there any...
04 May 2014 3,791 10 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?
15 July 2024 1,598 3 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...
10 July 2024 6,830 1 View
Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...
10 July 2024 4,918 2 View