Does IDPs have higher sensitivity in NMR compared to structured proteins?

Due to high flexibility IDPs expected to have higher sensitivity in NMR compared to structured proteins. Is this hypothesis correct? If yes can you suggest a suitable reference for this?

04 May 2019 8,689 2 View

Which buffer to use for NMR study peptide - Zinc interaction?

I am studying interaction of zinc with a peptide. Which buffer should I use ? And what is the highest concentration of zinc can used ?

08 September 2018 1,344 3 View

What is the protocol for monitoring Abeta (1-40) aggregation by ThT fluorescence assay ?

I want to study aggregation kinetics of Abeta (1-40) by ThT. I would like to know the working protocol for this study. I am using plate-reader based assay.

08 September 2018 5,069 0 View

How to compare stability of a protein and its mutant with NMR spectroscopy?

How can we compare stability of two proteins with the help of NMR spectroscopy? what are the traditional NMR techniques that we use for this purpose?

11 December 2017 3,626 4 View

What are the properties to be considered while designing a peptide for preventing Amyloid beta aggregation ?

If I design a peptide for preventing aggregation of Abeta 42 peptide, what are aspects should I consider if I want to suggest it as drug for Alzheimer disease. What all are the properties should...

10 November 2017 3,146 2 View

How to check presence of calcium in a protein sample?

I have a calcium binding protein. To make it calcium free i did dialysis with EDTA containing buffer. But how to make sure whether a protein is calcium free ?

10 November 2016 5,484 2 View

Can any one suggest some good reviews on Calcium binding proteins (especially crystallins) ?

In search of some review article on calcium binding proteins and exploring studies that can be performed on them.

05 June 2016 7,639 1 View

What are the disadvantages of CellSeearch System for capturing CTC ?

I read about circulating tumor cells and understood that cellsearch (Janssen) is the only FDA approved method that existing for capturing cancer cells and they are based on antigen-anibody...

10 November 2015 4,501 7 View

Why is DH5alpha better than BL21 for plasmid isolation?

Why do we generally prefer cells like DH5alpha or top10 for increasing plasmid amount and isolation? Why are we using BL21 for protein expression? Why not DH5alpha?

10 November 2014 3,194 10 View

How to express a protein with N-terminal cysteine?

I have a protein of 277 aminoacids. I want express a truncated version of that without first 22 aminoacids from N-terminal and also the sequence should start with a cysteine residue. Is there any...

04 May 2014 3,651 10 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How do you write a simplified C block code of this average-current control on PSIM?

This is a Buck (step-down) Converter and I am new to this program. I need to program with simplified C block and I actually don't know what to do. Can anyone show me how to do this? Or at least...

02 March 2021 8,233 1 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View