Does IDPs have higher sensitivity in NMR compared to structured proteins?

Due to high flexibility IDPs expected to have higher sensitivity in NMR compared to structured proteins. Is this hypothesis correct? If yes can you suggest a suitable reference for this?

04 May 2019 8,689 2 View

Which buffer to use for NMR study peptide - Zinc interaction?

I am studying interaction of zinc with a peptide. Which buffer should I use ? And what is the highest concentration of zinc can used ?

08 September 2018 1,344 3 View

What is the protocol for monitoring Abeta (1-40) aggregation by ThT fluorescence assay ?

I want to study aggregation kinetics of Abeta (1-40) by ThT. I would like to know the working protocol for this study. I am using plate-reader based assay.

08 September 2018 5,069 0 View

How to compare stability of a protein and its mutant with NMR spectroscopy?

How can we compare stability of two proteins with the help of NMR spectroscopy? what are the traditional NMR techniques that we use for this purpose?

11 December 2017 3,626 4 View

What are the properties to be considered while designing a peptide for preventing Amyloid beta aggregation ?

If I design a peptide for preventing aggregation of Abeta 42 peptide, what are aspects should I consider if I want to suggest it as drug for Alzheimer disease. What all are the properties should...

10 November 2017 3,146 2 View

How to check presence of calcium in a protein sample?

I have a calcium binding protein. To make it calcium free i did dialysis with EDTA containing buffer. But how to make sure whether a protein is calcium free ?

10 November 2016 5,484 2 View

What are the disadvantages of CellSeearch System for capturing CTC ?

I read about circulating tumor cells and understood that cellsearch (Janssen) is the only FDA approved method that existing for capturing cancer cells and they are based on antigen-anibody...

10 November 2015 4,501 7 View

Why is DH5alpha better than BL21 for plasmid isolation?

Why do we generally prefer cells like DH5alpha or top10 for increasing plasmid amount and isolation? Why are we using BL21 for protein expression? Why not DH5alpha?

10 November 2014 3,194 10 View

Any software for determining torsional angles from protein crystal structure???

08 September 2014 3,706 3 View

How to express a protein with N-terminal cysteine?

I have a protein of 277 aminoacids. I want express a truncated version of that without first 22 aminoacids from N-terminal and also the sequence should start with a cysteine residue. Is there any...

04 May 2014 3,651 10 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the value of Conference Review Paper?

How much value does a review paper carry in a higher study aspirant student's profile which is presented in a Peer Reviewed Scopus Indexed conference?

27 February 2021 2,068 3 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

How can Gamma Ray API units be converted to CPS (counts per second)?

Oil and gas companies run gamma ray tool and take readings in API units while Gamma ray is also noted in cps, so how these units can be converted into each other?

24 February 2021 7,545 2 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

Can't recognize exact phase and PDF card no of Silicon carbide in XRD?

We have deposited silicon carbide on carbon substrate using one PVD technique. Due to amorphous behavior of SiC at low deposition temperature, we performed heat treatment of those films at...

22 February 2021 2,072 3 View