Sujeesh Sukumaran

13 Questions 2 Answers 0 Followers

Questions related from Sujeesh Sukumaran

Does IDPs have higher sensitivity in NMR compared to structured proteins?

Due to high flexibility IDPs expected to have higher sensitivity in NMR compared to structured proteins. Is this hypothesis correct? If yes can you suggest a suitable reference for this?

05 May 2019 8,748 2 View

Which buffer to use for NMR study peptide - Zinc interaction?

I am studying interaction of zinc with a peptide. Which buffer should I use ? And what is the highest concentration of zinc can used ?

09 September 2018 1,402 3 View

What is the protocol for monitoring Abeta (1-40) aggregation by ThT fluorescence assay ?

I want to study aggregation kinetics of Abeta (1-40) by ThT. I would like to know the working protocol for this study. I am using plate-reader based assay.

09 September 2018 5,136 0 View

How to compare stability of a protein and its mutant with NMR spectroscopy?

How can we compare stability of two proteins with the help of NMR spectroscopy? what are the traditional NMR techniques that we use for this purpose?

12 December 2017 3,735 4 View

What are the properties to be considered while designing a peptide for preventing Amyloid beta aggregation ?

If I design a peptide for preventing aggregation of Abeta 42 peptide, what are aspects should I consider if I want to suggest it as drug for Alzheimer disease. What all are the properties should...

11 November 2017 3,203 2 View

How to check presence of calcium in a protein sample?

I have a calcium binding protein. To make it calcium free i did dialysis with EDTA containing buffer. But how to make sure whether a protein is calcium free ?

11 November 2016 5,544 2 View

Can any one suggest some good reviews on Calcium binding proteins (especially crystallins) ?

In search of some review article on calcium binding proteins and exploring studies that can be performed on them.

06 June 2016 7,692 1 View

What are the disadvantages of CellSeearch System for capturing CTC ?

I read about circulating tumor cells and understood that cellsearch (Janssen) is the only FDA approved method that existing for capturing cancer cells and they are based on antigen-anibody...

11 November 2015 4,579 7 View

Why is DH5alpha better than BL21 for plasmid isolation?

Why do we generally prefer cells like DH5alpha or top10 for increasing plasmid amount and isolation? Why are we using BL21 for protein expression? Why not DH5alpha?

11 November 2014 3,540 10 View

Any software for determining torsional angles from protein crystal structure???

I want to measure torsional angles from a protein crystal structure. Which is the most suitable software for that. I am not a biologist and not an expert in programming.

09 September 2014 3,759 3 View

How to express a protein with N-terminal cysteine?

I have a protein of 277 aminoacids. I want express a truncated version of that without first 22 aminoacids from N-terminal and also the sequence should start with a cysteine residue. Is there any...

05 May 2014 3,755 10 View

Why do we measure OD at 600 for understanding cell growth in bacterial culture?

Why not any other wavelength?

04 April 2014 9,742 1 View

I have purified a protein of 60 KD under denaturing condition using urea. Can anybody suggest a protocol to refold it to native state?

My protein is stable in 150mM NaCl and 20mM Tris. I tried refolding it by dialysis using this buffer but it didn't work.

01 January 2014 576 7 View