Dear Sir. Concerning your issue about the protocol for extraction of protoplast in mushrooms. I think the following below links may help you in your analysis:

https://www.tandfonline.com/doi/pdf/10.1271/bbb.60165

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769551/pdf/mb-34-73.pdf

http://www.academicjournals.org/article/article1380124215_Li%20et%20al%20(a).pdf

Thanks

Dear Sir. I have attached a protocol for extraction of protoplast in mushrooms. I think it will be helpful in your work.

Thank you Dr. Isam and Hafizur