Is it necessary to add PBS in Hoechst stain for making dilution?

01 January 1970 1 5K Report

Plant physiologist, Pathologist, Breeders, Botanist, Zoologist etc

Hello Anupam

Which Hoechst stain you are asking about? Secondly you are asking about making the working solution or dissolving the solid dye to make stock solution?

Its depends on the type of hoechst, depend on company also. However, solid can be dissolve in DMSO and then working solution can be made in PBS.

Regards.....

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