What is the proper in vitro study that allows me detect long term antitumor activity of an adenovirus construct?

Shahinaz Mahmood Shalaby @Shahinaz_Shalaby

01 January 1970 1 2K Report

If i am trying to find an antitumor activity of certain adenovirus construct, and this antitumor activity has been observed in vivo ...after exposure of tumor cells to the virus , they behaved less aggressively...How to design a study to go back and detect this effect in vitro?

Yusuf Haggag

Dear Dr Shahinaz,

I think, You should start first with cell viability assay (MTT assay) can be done for 5 days or more under certain conditions. This might help you for long term anti-tumor activity.

After that you should go for apoptosis assay or cell cycle analysis for confirmation.

There is also colony formation assay for more than one week to detect the anti-tumour activity of your treatment.

I hope you find an answer to your questions

Best of Luck

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