What is the promoter of pGEX exactly?

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Goodwin G. Jinesh

pGEX vectors such as, pGEX-3X and pGEX-2T, contains the Ptac (trp/lac) hybrid promoter, which is IPTG responsive. When IPTG is given the lac repressors dissociate from Ptac promoters and allowing transcription from promoter downstream sequences.

Kai Samuel Yang

Thanks a lot. I think it is a little different from other plasmids, such as pET and pQE.

Joanne Kamens

Can always look for backbone information on Vector Database

http://www.addgene.org/vector-database/2866/