Hello. I am confused on how to prepare quercetin standard curve. What is the general idea of preparing standard curve?
How should I decide what concentration and appropriate dilution used to plot a standard curve?
Thank you.
Dear Afrina Zaina,
if you are using quercetin for the estimation of its homologous/derivative from the plant or fruit materials you have to preparation caliberation standards 2-1000 ppm but if you are going to run it on HPLC for individual determination of quercetin then caliberation curve covering 2-500 microgram/mL would be sufficient.
Best of uck
Dear Afrina,
in our country we have i.v. form of quercetin as medications with stable dose. Please see my page on RG.
I am confused too. but when i searched i knew that the solvent is methanol and should prepare with serial dilution
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