Need the detailed protocol for the quantitative estimation of Glucosinolates in Cruciferous vegetable extracts.
Glucosinolate estimation
1. 500 mg Sample + 5ml distill water, homogenization
2. Make up volume up to 10ml
3. Kept for extraction in for 1hr on shaker
4. Add 5ml 20% TCA
5.Centrifuge @ 3000 rpm for 5min, collect the supernatant
6. To 1.5ml of supernatant + 1.5ml Ferrous nitrate reagent
7. Read O D @ 460nm within 2min after the addition of Ferrous nitrate reagent
Thank you sir. Procedure will be useful. Kindly send the reference for the procedure.
Your Welcome and Sure I will send reference paper..... soon...
Dear Jamuna... pls find the title of paper which contains details of glucosinolate...
Article Variations in the thiocyanate content of kale varieties
sir I would like to know how to prepare the standard graph as the fulltext of the paper is not available..
contact Dr. Elke Bloem at [email protected]
Please have a look at these useful links.
http://shodhganga.inflibnet.ac.in/bitstream/10603/147270/12/12_chapter_05.pdf
Article A Simple Spectrophotometric Method for Estimating Total Gluc...
Article A Straightforward Method for Glucosinolate Extraction and An...
http://orgprints.org/5611/1/5611.pdf
https://www.researchgate.net/post/i_want_the_total_methods_of_glutathione_and_Glucosinolate_estimation
The stress-strain calculations, energy density GI index calculations are also being done.
02 May 2024 9,963 4 View
Hi everyone...am a research scholar...doing research about behavior of thinwalled structures ...While doing nonlinear analysis in Abaqus... I got error msg as shown in figure... How to resolve...
02 February 2022 9,726 4 View
some researcher are confusing in methods of sampling with method of data collection I know the difference but still kindly share yours views.
03 March 2020 9,004 4 View
Few researchers are just showing sample taken without giving any reference to population does such studies are valid.
03 March 2020 897 4 View
Dear Researcher, I have used standards items from Burger(2009). Since in it all items where not their, therefore, I added few items based on literature reviews and got alpha value 0.9 which...
13 February 2020 7,850 5 View
Possibility of manufacturing IC engine connecting rod with Metal 3D printing technology? *If so how the mechanical properties will be while comparing Metal 3D-Printed connecting rod vs Forged...
11 June 2019 8,213 7 View
questionnaire contains 25 items in a single group.
28 March 2019 428 5 View
This has been bothering me for a while. In some of the design notes of power management ICs , it is mentioned that peak current mode control has 'lower gain - wider bandwidth' inner loop compared...
15 December 2018 1,791 2 View
I am working on FAN algorithm. The total number of samples in the reconstructed signal is only 50% of the original signal. The compression part is working properly. I request you to kindly guide...
25 September 2017 8,787 0 View
I am currently culturing the T-CLL, Kit-225 cell line. My culture condition is RPMI 10% FCS with 20ng/ml IL2 (R&D, about 420IU/ml). Cells look viable but proliferation rate is slow. Is there...
27 June 2017 2,030 6 View
Approximate concentrations are require in compared with the WHO permissible limts
11 August 2024 2,723 1 View
Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...
09 August 2024 5,444 7 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...
06 August 2024 4,849 2 View
Air moisture harvesting Air water collection devices
06 August 2024 5,473 2 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
Are increasing the costs of disasters in the affected countries.
01 August 2024 1,794 2 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View