I Can we use a modified version of standards items ?

Dear Researcher, I have used standards items from Burger(2009). Since in it all items where not their, therefore, I added few items based on literature reviews and got alpha value 0.9 which...

13 February 2020 7,661 5 View

How to determine sample size for questionnaire development ?

questionnaire contains 25 items in a single group.

28 March 2019 309 5 View

The best culture condition for Kit-225

27 June 2017 1,898 6 View

How can I overcome such problematic melt curve in qPCR?

01 January 2016 8,023 4 View

How to achieve better amplification in qPCR?

30 October 2015 4,000 8 View

If the data collected is classified into more number of classes, will it improve the accuracy of goodness of fit test? Why?

This question is with regards to the accuracy of goodness of fit test.

04 January 2015 7,404 3 View

For a standard second order transfer function, what is the equivalent time domain significance of Zeta >0.707?

Zeta>0.707 implies no resonant peak in frequency response. But I am unable to correlate what is the exact time domain effect of this? If Zeta= 0.707 acts like an 'important damping value' in...

09 December 2014 683 11 View

What is the procedure for isolation of myrosinase enzyme from cruciferous vegetable extracts?

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31 July 2014 5,105 3 View

Can formalin mix with Triton-X in PBS to make 3.7% formalin with 0.1% Triton-X solution for intracellular staining?

A standard protocol for intracellular staining usually separate the fixation and permeabilization part. Can I do the fixation and permeabilization at one time by incubating cells with 3.7%...

16 July 2014 8,541 5 View

What is the difference between non-fat milk and BSA for primary antibody in western blot?

Some protocols mention primary antibodies should be diluted in 5%BSA whereas some protocols mention primary antibodies should be diluted in 5% non-fat milk. What is the difference? What is the...

25 May 2014 8,114 19 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View