My question is about synthesis of nanostructure. the drug is doxycycline monohydrate. nanostructure consists of micelle TPGS and plunoric acid (f127).
at first stage, preparation of drug is needed and i used water and ethyl acetate(aqueous and organic solvent). the purpose of the stage is acid base extraction. I don't know if the solvents are good or not because doxycycline monohydrate very little dissolved in water and sonication used. stage 2 is synthesis of 40 mg micelle TPGS + 60mg f127 and i used ethanol (as a solvent). 2 mg drug added to solution. Rotary(30 min) and freeze dryer(2h) used and then 5mL PBS added. for 24h under the stirrer temperature was 37 centigrade. after that DLS was taken from the sample. but result is not good. two peaks showed and PDI is 0.4. z-average is about 24 nm. what is the problem in stages?
Fatemeh Bagherzadeh The issues in your nanostructure synthesis process may stem from the choice of solvents and drug loading efficiency. Specifically, the low solubility of Doxycycline monohydrate in water during acid-base extraction and the heterogeneity observed in the micelle size (as indicated by two peaks in DLS data) could be contributing factors.
Ethanol is commonly used for micelle preparation, but it should not negatively affect drug stability or micelle formation. Consider optimizing solvent selection and micelle formation to improve your results.
- Patel, H. S., Vyas, B. A., Tripathi, S., & Sharma, R. K. (2023). Design, Development, and Evaluation of SA-F127: TPGS Polymeric Mixed Micelles for Improved Delivery of Glipizide Drug: In-vitro, Ex-vivo, and In-vivo Investigations. AAPS PharmSciTech, 24(8), 213.
- Zhang, M. L., Zhang, G. P., Ma, H. S., Pan, Y. Z., & Liao, X. L. (2024). Preparation of pH-responsive polyurethane nano micelles and their antibacterial application. Journal of Biomaterials Science, Polymer Edition, 1-16.
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