Typical concentrations of glucose transporters in mouse brain?

I have measured levels of GLUT1, GLUT3, and GLUT4 in the mouse hippocampus and cortex using ELISA kits. I'm trying to find typical concentrations (in ng/mg total protein) of these proteins in the...

21 December 2020 4,647 2 View

Is passage number of cell line crucial for developing leukemia in SCID mice?

Hi everyone, I am trying to develop leukemia in NOD SCID mice with BCR/ABL over expresssed cell line. But somehow the mice is not showing any signs of the disease. The passage number of cell line...

25 October 2020 5,614 3 View

I want to know the BET surface area of nano-rod shaped ZnO nano-particles?

Anyone if you know the BET surface area of nano-rod shaped ZnO nano-particles than please comment. The nano rod-shaped ZnO nanoparticles are synthesized by chemical precipitation method. If...

25 May 2020 8,856 1 View

What could be the reason for a fiber shape TiO2 nanowire besed dye sensitized solar not to work?

I am trying to fabricate a fiber shape TiO2 nanowire-based dye-sensitized solar cell on Ti wire. I use a hydrothermal route to grow the TiO2 nanowires. Then I absorb the nanowires with N719 dye. I...

15 December 2019 1,407 3 View

How to decompose a backbone curve into 10 elasto plastic parallel springs in abaqus?

Presently I am trying to implement the SSI technique on a pile on clay soil by attaching non-linear spring decomposed from the backbone curve for my master's project. can anyone help me how to...

15 October 2019 905 1 View

Can I use a sonicator to clean Ti wire?

I want to use Ti wire to make fiber shape solar cell. In literature they use an ultra-sonicator to clean Ti wire. Can I use a sonicator for this purpose as I do not have an ultra sonicator...

22 August 2019 5,066 9 View

Can i uncoat my gelatin from cell culture flask?

I have coated my culture flask with more concentrated gelatin than required accidentally. I haven't seeded my cells yet. Can i uncoat them and again coat them with desired concentration?

24 April 2019 529 1 View

Can i do immunofluorescence in cover slip for my HUVEC? If so, how?

I am going to do immunofluorescence from my cultured HUVEC. Before doing IF test i need to treat my cells with my test serum sample for 6 hours. I don't have chamber slide for this. So I am in...

08 April 2019 3,948 3 View

DNA analysis in glass microfluidic channel has showed a lot of sticking problem and eventually clog the channel. Any solution to reduce DNA sticking?

I am doing DNA separation in micro-fluidic channel network made of glass. There I see DNA sticking on glass channel wall that impedes DNA movements and eventually clog the channel. After few...

19 March 2019 6,839 3 View

How to preserve and process endocervical swab for cytokin analysis by flow cytometry?

I wanna analysis cytokines level in different stages of CIN and ca cervix both in HPV DNA positive and negative cases. We collected 1000microliter cervical swab by cyto brush for detection of HPV...

12 March 2019 2,628 2 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View