Although we can complete our protocol with other strains, we confront the same problem over over again with same patient. Picture of PFGE is attached. The left one belongs to another patient and is acceptable. But what is wrong with other two lines?
You will have to be more specific than "what is the problem" . Is the left hand sample digested with enzyme?or is it a marker ? It looks as if it comes from a separate gel altogether and has been pasted in . If it is a sample, where are your markers so that we have some idea of the size we are looking at. with? What sort of patient sample?Is it tissue - eg biopsy or a body fluid sample eg blood ,sputum. How was it collected, how was it purified ? In gel digestion? Digested and then loaded? Overnight run at 4C ?You mention strains -of what - bacteria? virus?
It looks like a pair of degraded samples of some sort but we need more information to be able to help.
Ian
Could be
*incomplete Cell lysis
*a failure in the restriction enzyme digest
*contaminated Pfge electrophoresis- DNAse activity
*non typable strains ( could become typable by the addition of Thiourea to the running buffer)
What species was investigated shown in the picture?
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