Analysis of CRD two factor using R studio ?

Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...

28 February 2021 8,189 3 View

What is the role of work experience in motivating high tech entrepreneurship in Aviation industry?

Related work experience plays a vital role in motivating the Entrepreneurship. But when we talk about high tech entrepreneurship in Aviation industry, we talk about very high investment and high...

22 February 2021 376 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How doping in nano-particles with same valency affect it's crystal lattice structure?

When doping in case of semiconductor is concerned, we doped Si by trivalent or pentavalent impurity atoms to improve its electronic properties but in case of nano-particles, sometimes we doped...

31 January 2021 7,128 1 View

How to prevent aggregation of nano-particles without using surfactants?

As it is well known fact that nano-particles can easily aggregate during nucleation/ synthesis part due to high surface energy..by reading literature, I came to know that surfactants can help to...

30 January 2021 8,206 6 View

How do I remove tetrabutylammonium perchlorate after electrolysis of organic molecules?

I am doing electrochemical oxidation of organic compounds for the the synthesis of nitrocompounds from simple organic molecules. I want remove supporting electrolyte after electrolysis. Please...

03 January 2021 7,858 2 View

Why the errors like the ones uploaded below happens while using Meta R/ Adel R?

Greetings everyone, I am learning to analyze multi-year and environment crop data using Meta R/ Adel R. But at some point during the process some errors were continuously observed and I could not...

18 December 2020 6,285 7 View

What is the best quality DNA for SSR fingerprinting?

Nanodrop result of my DNA samples extracted from fresh finger millet leaves showed 260/280 ratio ranged from 1.7-2.2 whereas 260/230 ratio ranged from 1.1-2.2. How can I assess the quality of such...

30 November 2020 8,689 6 View

Why Schiff bases change colour upon evaporation?

I got bright yellow coloured Schiff base after 6 hour reaction time. So i kept it for slow evaporation. Solvent was methanol. But after complete evaporation my product colour changes from yellow...

17 November 2020 3,824 2 View

How extract and purify extracellular proteins expressed in E. coli?

Hello everyone, I am having trouble isolating and purifying an extracellular protein which I have expressed in E.coli. I have induced my protein at 0.5 mM IPTG by incubating it at 37 degrees for...

02 October 2020 8,223 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View