pka of direct orange 34?
I question about the appropriate journal to publish our findpings. My colleagues have published a discovery in a low impact journal. After reading the publication, I swear they can publish their...
05 June 2018 7,792 2 View
Researchers are doing hard works on controlling the preparation of nanomaterials that would allow higher efficiency as nanoadsorbents. I question how can we prepare a "flower-like" or...
04 May 2018 7,630 4 View
What is the best way to increase visibility? The author can publish many papers in only one journal or select different one each time he/she submit his new manuscript.
04 May 2018 7,949 4 View
A Suggested Procedure for Determining Order of Authorship..!!!
31 December 2016 8,115 5 View
_average publication for science and engineering researcher.
09 October 2016 4,712 15 View
Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?
01 March 2021 9,447 3 View
Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...
26 February 2021 9,119 3 View
Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...
19 February 2021 472 5 View
Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...
17 February 2021 5,399 1 View
Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...
08 February 2021 3,366 3 View
Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...
07 February 2021 9,989 7 View
I was doing MTT assay, and in the last day before adding the MTT; the cells looked good (or so I think), but after aspiration of the drug and addition of MTT dye I noticed that most cells seemed...
07 February 2021 3,846 4 View
Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...
04 February 2021 4,482 4 View
Hi guys I'm out of ideas here please help! I've never had consistent problem like this doing westerns before and I can't figure it out. The condition used: - First 4 lanes have 50ug protein in...
04 February 2021 2,297 3 View
Hi I have a protein labeled with rhodamine B and after reaction i used Zeba spin column for dye removal. my problem is that lot of unreacted dye remained which interfered with my florescent...
02 February 2021 644 2 View
